A DEVELOPED SPECTROPHOTOMETRIC DETERMINATION OF AMOXICILLIN FORMS VIA CHARGE- TRANSFER REACTION WITH METOL

AA ssppeeccttrroopphhoottoommeettrriicc mmeetthhoodd ffoorr ddeetteerrmmiinnaattiioonn ooff aammooxxiicciilllliinn ((AAMMXX)) iinn ppuurree aanndd pphhaarrmmaacceeuuttiiccaall ffoorrmmuullaattiioonnss iiss ddeessccrriibbeedd.. TThhee pprrooppoosseedd mmeetthhoodd iinnvvoollvveess tthhee uussee ooff mmeettooll ((NN--mmeetthhyyll--pp--hhyyddrrooxxyy aanniilliinnee)) aass aa cchhrroommooggeenniicc rreeaaggeenntt..TThhee ddrruugg pprroodduuccee aa bblluuiisshh--ggrreeeenn ccoolloouurreedd ssoolluubbllee cchhaarrggee- - ttrraannssffeerr ccoommpplleexx wwiitthh mmeettooll iinn tthhee pprreesseennccee ooff ppoottaassssiiuumm ppeerrssuullpphhaattee iinn aallkkaalliinnee mmeeddiiuumm.. The coloured product is measured at λmax 620 nm . Under the optimum conditions calibration graph was observed a linear from 5-60 μg ml-1 AMX and with correlation coefficient not less than 0.9994 which was suitable for the quantitative determination of the drug.The molar absorbitivity was 2.726×103 L.mole-1.cm-1,and sandell sensitivity of 0.1538 μg cm-2 . The relative standard deviation of the proposed method was 0.25-2.44% depending on the concentration of AMX. The proposed method was successfully applied to the determination of AMX in injections and capsules.