Author/Authors :
Hajjaran, H. tehran university of medical sciences tums - School of Public Health and Institute of Public Health Research - Dept of Medical Parasitology Mycology, تهران, ايران , Azarian, B. Pasteur Institute of Iran - Biotechnology Research Centre - Protein Chemistry Unit, ايران , Mohebali, M. tehran university of medical sciences tums - Faculty of Medicine - Dept of Medical Parasitology and Mycology, تهران, ايران , Hadighi, R. tehran university of medical sciences tums - School of Medicine - Department of Medical Parasitology and Mycology, تهران, ايران , Assareh, A. tehran university of medical sciences tums - School of Public Health - Department of Medical Parasitology and Mycology, تهران, ايران , Vaziri, B. Pasteur Institute of Iran - Biotechnology Research Centre - Protein Chemistry Unit, ايران
Abstract :
In order to define the protein expressional changes related to the process of meglumine antimoniate resistance in anthroponotic cutaneous leishmaniasis (CL), we performed a comparative proteomics analysis on sensitive and resistant strains of Leishmania tropica isolated from Iranian CL patients. Cell proteins were analysed with 2-dimensional electrophoresis and differentially expressed proteins were identified by matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry. Image analysis of the matched maps identified 7 proteins that were either over- or down-expressed: activated protein kinase c receptor (LACK), alpha tubulin (×2), prostaglandin f2-alpha synthase, protein disulfide isomerase, vesicular transport protein and a hypothetical protein. The study shows the usefulness of proteomics in identifying proteins that may express differences between sensitive and resistant L. tropica isolates.
