Glycation inhibition activity of 5-Sulfosalicyclic acid in diabetic condition

Objective: To study the glycation inhibition effect of Sulfosalicyclic acid. Methodology: The study was conducted by using plasma from apparently healthy non diabetic and diabetic persons. Samples of different concentrations of glucose and inhibitor were incubated for 5 weeks at 37oC temperature. Glucose was estimated by glucose oxidase kit method before and after dialysis. Glycation level was assessed and measured by TBA and periodate assays. Results: Increase in glycation was observed from 1st to 3rd week of incubation while it was decreased after 5th week due to formation of advanced glycation end products. Three different concentrations of inhibitor showed variable responses. It was also noted that 10 mM concentration of Sulfosalicyclic acid exhibited fairly good response to decrease glycation. Conclusions: Highest concentration of Sulfosalicyclic acid produced overall high level of inhibition. Periodate borohydride proved to be more reliable and sensitive glycation assay when compared to TBA test.