Author/Authors :
Taheri -Anganeh, Mortaza Depa rtment of Medical Biotechnology - School of Advanced Medical Sciences and Technologies - Shiraz University of Medical Sciences, Shiraz, Iran , Amiri, Ahmad Department of Biochemistry - School of Medicine - Shiraz University of Medical Sciences, Shiraz, Iran , Movahedpour, Ahmad Depa rtment of Medical Biotechnology - School of Advanced Medical Sciences and Technologies - Shiraz University of Medical Sciences, Shiraz, Iran , Khatami, Hossein Depa rtment of Medical Biotechnology - School of Advanced Medical Sciences and Technologies - Shiraz University of Medical Sciences, Shiraz, Iran , Ghasemi, Younes Department of Pharmaceutical Biotechnology - School of Pharmacy - Shiraz University of Medical Sciences, Shiraz, Iran , Savardashtaki, Amir Amir Savardashtaki1Depa rtment of Medical Biotechnology - School of Advanced Medical Sciences and Technologies - Shiraz University of Medical Sciences, Shiraz, Iran , Mostafavi -Pour, Zohreh Department of Biochemistry - School of Medicine - Shiraz University of Medical Sciences, Shiraz, Iran
Abstract :
Breast cancer is one of the most prevalent cancers among women. Common cancer treatment methods are not effective enough, and there is a need for a more efficient treatment procedure. Cancer vaccine is a novel immunotherapy method that stimulates humoral and/or cellular immunity against cancer. Placenta-specific protein 1 (PLAC1) is a cancer/testis antigen, prevalent in breast cancer and rarely found in normal tissues. FliC, as a bacterial adjuvant, when fused to PLAC1 can elicit humoral and cellular responses. Therefore, PLAC1-fliC is a chimeric protein, which can be considered a suitable candidate against breast cancer. Methods: ProtParam was used to evaluate the physicochemical properties of PLAC1-fliC. Second structures were determined using the GOR V server. PLAC1-fliC 3D structure was modeled by Phyre2, and it was refined using GalaxyWEB. The refined model was submitted to RAMPAGE, PROCHECK, and ProSA-web for validation. Antigenicity and allergenicity of the construct were predicted by ANTIGENpro, VaxiJen, AllergenFP, and SDAP databases. Then MHC-I- and MHC-II-binding epitopes of PLAC1-fliC were forecasted by NetMHC 4.0 and NetMHCII 2.3 Servers. Finally, Ellipro and CTLpred were employed to predict B-cell and cytotoxic T lymphocyte epitopes. Results: The construct was evaluated as a stable fusion protein, which could be antigenic and could stimulate B and T cells against breast cancer. Conclusion: PLAC1-fliC, as a cancer vaccine candidate, might be suitable and specific for breast cancer, which could evoke humoral and cellular immunity against this type of tumor.
Keywords :
Breast cancer , PLAC1 , Bioinformatics , Cancer vaccines
