Title of article :
Enhanced Adipose Mesenchymal Stem Cells Proliferation by Carboxymethyl-Chitosan Functionalized Polycaprolactone Nanofiber
Author/Authors :
Shapourzadeh, Atena Department of Biochemistry - Faculty of Basic Science - Islamic Azad University Damghan Branch, Damghan, Iran , Atyabi, Mohammad Department of Pilot Nanobiotechnology - Pasteur Institute of Iran , Irani, Shiva Science and Research Branch Islamic Azad University, Tehran, Iran , Bakhshi, Hadi Universitatsstraße Bayreuth - Germany -Universitatsstraße Bayreuth, Germany
Abstract :
Background: Through combining two synthetic and natural polymers, scaffolds can be developed for tissue engineering and regenerative medicine purposes. Methods: In this work, carboxymethyl chitosan (CMC; 20%) was grafted to Polycaprolactone (PCL) nanofibers using the cold atmospheric plasma of helium. The PCL scaffolds were exposed to CAP, and functional groups were developed on the PCL surface. Results: The results of Fourier Transform Infrared Spectroscopy confirmed CMC (20%) graft on PCL scaffold. The Thiazolyl blue tetrazolium bromide assay showed a significant enhancement (p < 0.05) in the cell affinity and proliferation of adipose-derived stem cells (ADSCs) to CMC20%-graft-PCL scaffolds. After 14 days, bone differentiation was affirmed through alizarin red and calcium depositions. Conclusion: Based on the results, the CMC20%-graft-PCL can support the proliferation of ADSCs and induce the differentiation into bone with longer culture time.
Keywords :
Carboxymethyl chitosan , Mesenchymal stem cells , Tissue engineering
Journal title :
Iranian Biomedical Journal(IBJ)
