Author/Authors :
Kassaa, I. Lebanese University - AZM Centre for Biotechnology Research and its Applications, Doctoral School of Sciences and Technology - Laboratoire de Microbiologie Santé et Environnement, Lebanon , Hamze, M. Lebanese University - Faculty of Public Health, AZM Centre for Biotechnology Research and its Applications, Doctoral School of Sciences and Technology, - Laboratoire de Microbiologie Santé et Environnement, Lebanon , Dabboussi, F. Lebanese University - Faculty of Public Health, AZM Centre for Biotechnology Research and its Applications, Doctoral School of Sciences and Technology - Laboratoire de Microbiologie Santé et Environnement, Lebanon , Mallat, H. Lebanese University - AZM Centre for Biotechnology Research and its Applications, Doctoral School of Sciences and Technology, Faculty of Public Health - Laboratoire de Microbiologie Santé et Environnement, Lebanon , Mallat, H. Nini Hospital Laboratory, Lebanon , Achkar, M. Nini Hospital Laboratory, Lebanon , Hlais, S. Lebanese University - AZM Centre for Biotechnology Research and its Applications, Doctoral School of Sciences and Technology, Faculty of Public Health - Laboratoire de Microbiologie Santé et Environnement, Lebanon , Hlais, S. Saint-Joseph University, Lebanon
Abstract :
The aim of this study was to determine the capsular typing and type b prevalence of clinical Haemophilus influenzae strains in north Lebanon in both invasive and non-invasive disease and to determine the susceptibility pattern and the mechanism of resistance to β-lactams [β-lactamase–producing strains and β-lactamase–negative ampicillin resistant (BLNAR) strains]. Fifty-two strains of clinical H. influenzae were isolated from 312 clinical specimens; the resistance pattern to β-lactams of these strains was determined by using the disc diffusion and E-test methods followed by molecular methods such as PCR of bla_TEM et bla_ROB genes. Nine (17.4%) of the 52 strains were resistant to ampicillin; all of them produced type TEM-1 β-lactamase. In the susceptible strains 15.3% were not fully susceptible to β-lactams or considered low BLNAR strains. Slide agglutination serotyping showed that 30.7% of the strains were type b.
