THE EFFECT OF DIFFERENT CULTURE MEDIA ON IN VITRO FERTILIZATION OF MICE

The effect of different types of media on in vitro fertilization (IVF) of mice oocyte was investigated. IVF was carried out in RPMI-1640 medium at recommended temperature (37° C) and pH (7.4). The obtained results showed that 63.73% of used oocytes were fertilized, and the percentage of abnormal embryos was 27.58%. While, using other culture media at same temperature and pH, gave low percentage of fertilization and higher yield of abnormal embryos. Thus, using Medicult IVF, Earl s and TCM-199 were gave fertilization percentages equivalent to 38.75, 42.42 and 61.45% respectively; whereas, the percentages of abnormal embryos were 32, 35.7 and 33.89 % respectively. The reported results indicate that composition of various types of culture media have significant effect on the rate of fertilization and abnormal embryos.