Genetic study to bacteria Acinetobacter baumannii - Lactamase producer

Six isolates were collected from different clinical sources from laboratory in medicine century. These isolates were belonging to the genus Acinetobacter depending on morphological and biochemical tests. The antibiotic susceptibility tests against 8 antibiotics were examined, and it was found that the all isolates have multiple resistant to antibiotic, all isolates were resistant to ampicillin, ceftriaxone, cefotaxime, 50% were resistant to augmentin, 20% were resistant to ciprofloxacin ,while 10% from isolates show resistant to amikacin and trimethprim. All isolates have sensitivity to imipenem. The ability of Acinetobacter isolates to produce -lactamase enzymes was, tested using iodometric method, and the results showed that all isolates produced this enzyme. The ability of these isolates to produce extended spectrum -lactamase (ESBLs) were also determined by double disc synergy test, only one isolates produced these enzyme. Agarose gel electrophoresis showed that Acinetobacter isolates -lactamase producer have two small plasmid bands. Transformation experiments revealed that these plasmids were capable to transform E. coli MM294, an observation which indicates the ability of these plasmids to show their expression in more than one host.