Author/Authors :
AKHTAR, NAVEED Islamia University of Bahawalpur - Faculty of Pharmacy and Alternative Medicine - Department of Pharmacy, Pakistan , AZIZ, GULZEB Islamia University of Bahawalpur - Faculty of Pharmacy and Alternative Medicine - Department of Pharmacy, Pakistan , AHMAD, MAHMOOD Islamia University of Bahawalpur - Faculty of Pharmacy and Alternative Medicine - Department of Pharmacy, Pakistan , MADNI, ASAD ULLAH Islamia University of Bahawalpur - Faculty of Pharmacy and Alternative Medicine - Department of Pharmacy, Pakistan , ASHRAF, MOHAMMAD Islamia University of Bahawalpur - Faculty of Pharmacy and Alternative Medicine - Department of Pharmacy, Pakistan , MAHMOOD, AHMED University of the Punjab - College of Pharmacy, Pakistan
Abstract :
A sensitive, accurate and rapid reverse phase HPLC method was developed to quantify plasma levels of famotidine in order to conduct a comparative bioavailability study. Famotidine was extracted by liquid-liquid extraction method and analyzed on a Hypersil ODS C18 (150 x 4.6 mm) column, with disodium hydrogen phosphate (20 mM), sodium dodecyl sulphate (50 mM) in distilled water (pH 3.0) and acetonitrile in the ratio of 70: 30 with UV-detector at 267 nm. The standard curvecovering 0.093-1.5 μg/ mL concentration range was linear (r^2 = 0.9986), CV ranged 1.34-5.83 forintra-day and 1.10-6.92 for inter-day variation. The method was applied for the determination ofcomparative bioavailability of the two formulations coated with different coating agents. There was astatistical significant difference (p 0.05) between area under concentration vs time curve of formulation 1 (F1) and formulation 2 (F2) and their values were 3.97 ± 1.61μg.h/ mL and 10.07 ±0.21μg.h/ mL, respectively.
