Title of article
Effect of Molecular Mutagenesis on Alcohol Dehydrogenase Production by Pseudomonas fluorescens RB30
Author/Authors
Hadi, Noora A. University of Al-Nahrain - College of Science - Department of Biotechnology, Iraq , Al-Gelawi, Majed H. University of Baghdad - College of Science - Department of Biotechnology, Iraq , Aziz, Ghazi M. Baghdad University - College of Science - Biotechnology Department, Iraq
From page
140
To page
144
Abstract
Alcohol dehydrogenases are group of dehydrogenase enzymes that occur in many organisms and facilitate the interconversion between alcohols and aldehydes or ketons with the reduction of NAD^+ to NADH. Locally isolated Pseudomonas fluorescens RB30 was selected depending on its efficiency in producing of alcohol dehydrogenase (ADH) enzyme. Its specific activity was 13.6 U/mg proteins after extraction by using lysozyme. As an attempt to improve ADH production, Pseudomonas fluorescens RB30 was subjected to molecular mutagenesis following conjugation with E. coli PULB113, Tet^r and Km^r harboring the conjugative plasmid RP4 that carried Mini-Mu transposon, and two mutants were due to their high activities were resulted (0.82 U/ml and 0.79 U/ml respectively).
Keywords
Pseudomonas , alcohol dehydrogenase , molecular mutagenesis
Journal title
Al-Nahrain Journal Of Science
Journal title
Al-Nahrain Journal Of Science
Record number
2646135
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