Author/Authors :
al-rawi, al-shaimaa muhammed saeed university of baghdad - college of sciences - department of biotechnology, Iraq , al-kazaz, abdul kareem a. university of baghdad - college of science - department of biotechnology, Iraq , sabbah, majeed arsheed al-nahrain university - forensic dna center, Baghdad, Iraq , al-zubaidi, mohammed mahdi al-nahrain university - forensic dna center, Baghdad, Iraq
Abstract :
Many research groups developed real time PCR assays for identification of Methicillin-resistant Staphylococcus aureus (MRSA). They designed different primers and probes in their assays. Gene mecA is the target identification of MRSA by PCR assay. The aim of this study is using in silico approach to identify the best primers and probe for real time PCR identification mecA gene. Published primers and probes were analyzed in silico to select the best for real time PCR identification of mecA gene. The selected primers and probe successfully used for real time amplification of twenty MRSA tested. This study reveals the importance of in silico approach for designing diagnostic assays shorten the cost and the time.
Keywords :
Methicillin , resistant Staphylococcus aureus (MRSA) , real time PCR , in silico
