Author/Authors :
SALIMI, MONA Pasteur Institute of Iran - Research Development Department, ايران , POURABDI, LADAN sharif university of technology - Faculty of Chemistry - Organic Chemistry Department, تهران, ايران , ZAKERI, MARYAM Pasteur Institute of Iran - Biotechnology Department, ايران , ABDIPOUR, NAZANIN Pasteur Institute of Iran - Research Development Department, ايران
Abstract :
A crude phenolic glycolipid extract from Mycobacterium bovis BCG was fractionated by column chromatography. A reversed-phase high performance liquid chromatography (HPLC) method with UV detection at 275nm was developed for simultaneous detection and separation of phenolic glycolipids (PGLs) in Mycobacterium bovis BCG. This analysis provides a good resolution. Different solvent systems and columns for HPLC were compared. A system composed of acetonitrile-water in the ratio of 0→80% at a flow rate of 0.8 mL/min and C8 analytical column were found to be optimum for HPLC of the phenolic glycolipids. This simple method is therefore appropriate to purify these compounds present in M. bovis extract.
Keywords :
HPLC , UV , mycobacterium , phenolic glycolipid
