Title of article :
THE INTRACEREBROVENTRICULAR (ICV) ADMINISTRATION OF W-7, A CALMODULIN INHIBITOR, ATTENUATES THE DEVELOPMENT OF MORPHINE TOLERANCE IN RATS
Author/Authors :
SEPEHRI, GHOLAMREZA kerman university of medical sciences - Neuroscience Research Center, ايران , SHEIBANI, VAHID kerman university of medical sciences - Neuroscience Research Center, ايران , AZARANG, AFROOZ kerman university of medical sciences - Neuroscience Research Center, ايران , SHAMSIZADEH, ALI rafsanjan university of medical sciences - Department of Physiology, ايران , AFARINESH, MOHAMMAD REZA kerman university of medical sciences - Neuroscience Research Center, ايران , AZIZOLLAHI, SAEED kerman university of medical sciences - Physiology Research Center, ايران , SEPEHRI, EHSAN tehran university of medical sciences tums, تهران, ايران
Abstract :
The present study was performed to determine the effect of intracerebroventricular (ICV) administration of W- 7, a specific calmodulin inhibitor, on the development of tolerance to antinociceptive effect morphine administration. This study was carried out on male wistar rats, weighing 200-250 g. Morphine was administered daily (15 mg/kg for 8 days). The threshold to thermal nociceptive stimuli was measured by tail-flick test. W-7 (0.25, 0.5 and 1 μmol/rat) was injected through ICV. Maximal possible effect percentage (MPE %) was considered as analgesia index. Our result showed that chronic morphine exposure induced tolerance to its antinociceptive effect and administration of W-7 (0.5 and 1 μmol/rat) decreased the development of tolerance to it. In conclusion these data showed that chronic injection of W-7 inhibited the development of morphine tolerance which indicates that calmodulin and its dependent pathways may play a role in the morphine tolerance processes.
Keywords :
Calmodulin inhibitor (W , 7) , morphine tolerance , rat
