Title of article :
ANTIOXIDANT ACTIVITY OF COVOLVULUS HYSTRIX VAHL AND ITS CHEMICAL CONSTITUENTS
Author/Authors :
DONIA, ABD EL RAHEIM MOHAMMED Desert Research Center - Medicinal and Aromatic Plants Department, Egypt , DONIA, ABD EL RAHEIM MOHAMMED Al Kharj University - College of Pharmacy - Pharmacognosy Department, Saudi Arabia , ALQASOUMI, SALEH IBRAHIM Al Kharj University - College of Pharmacy - Pharmacognosy Department, Saudi Arabia , AWAAD, AMANI SHAFEEK King Saud University - College of Science - Chemistry Department, Saudi Arabia , CRACKER, LYLE University of Massachusetts - Department of Plant Soil Insect Sciences, Saudi Arabia
Abstract :
Total alcohol and successive extracts (diethyl ether, chloroform, ethyl acetate and n-butanol) of Covolvulus hystrix Vahl were screened for their antioxidant activity using free radical 2-2- diphenyl-1-picrylhydrazyl (DPPH) and Trolox as reference substance. The tested extracts showed variable antioxidant activities. The highest antioxidant activity was achieved in total alcohol (48.32%) followed by ethyl acetate (37. 8 %) and nbutanol (32.96 %) extracts. From the ethyl acetate extract four compounds were isolated and identified as quercetin, quercetin 3-0-rutinoside and quercetin-7-O-rhamnoside in addition to ferulic acid using different spectroscopic analysis such as, 1HNMR, 13CNMR, COSY, HMBC and HMQC.
Keywords :
Antioxidant activity , Convolvulus hystrix , flavonoids , ferulic acid , total phenolics.
Journal title :
Pakistan Journal Of Pharmaceutical Sciences
Journal title :
Pakistan Journal Of Pharmaceutical Sciences
