Title of article :
EFFECTS OF MARMIN, A COMPOUND ISOLATED FROM AEGLE MARMELOS CORREA, ON CONTRACTION OF THE GUINEA PIG-ISOLATED TRACHEA
Author/Authors :
NUGROHO, AGUNG ENDRO Gadjah Mada University - Faculty of Pharmacy - Department of Pharmacology and Clinical Pharmacy, Indonesia , ANAS, YANCE Gadjah Mada University - Faculty of Pharmacy - Department of Pharmacology and Clinical Pharmacy, Indonesia , ARSITO, PUGUH NOVI Gadjah Mada University - Faculty of Pharmacy - Department of Pharmacology and Clinical Pharmacy, Indonesia , WIBOWO, JOKO TRI Gadjah Mada University - Faculty of Pharmacy - Department of Pharmacology and Clinical Pharmacy, Indonesia , RIYANTO, SUGENG Gadjah Mada University - Faculty of Pharmacy - Department of Pharmaceutical Chemistry, Indonesia , SUKARI, MOHAMAD ASPOLLAH Universiti Putra Malaysia - Faculty of Science - Department of Chemistry, Malaysia
Abstract :
Marmin or 7-(6’,7’-dihydroxygeranyl-oxy)coumarin is a compound isolated from Aegle marmelos Correa. In the study, we examined the effects of marmin on the contraction of guinea pig-isolated trachea stimulated by several inducers, namely histamine, metacholine, compound 48/80. We also evaluated its action against contraction induced by extracellular or intracellular calcium ion. The possibility of marmin to potentiate the relaxation effect of isoprenaline was also studied. Marmin added in the organ bath at 10 min prior to the agonist inhibited the contraction elicited by histamine and metacholine in a concentration-dependent manner. Moreover, marmin antagonized the histamine-induced contraction in competitive manner. Marmin mildly potentiated the relaxation effect of isoprenaline. In the study, marmin abrogated the contraction of tracheal smooth muscle induced by compound 48/80, an inducer of histamine release. Besides, marmin successfully inhibited CaCl2-induced contraction in Ca2+-free Krebs solution. Marmin also inhibited two phases of contraction which were consecutively induced by metacholine and CaCl2 in Ca^2+-free Krebs solution. Based on the results we concluded that marmin could inhibit contraction of the guinea-pig tracheal smooth muscle, especially by interfering histamine receptor, inhibiting the histamine release from mast, inhibiting intracellular Ca^2+ release from the intracellular store and the Ca^2+ influx through voltage-dependent Ca^2+ channels.
Keywords :
Aegle marmelos Correa , marmin , trachea smooth muscle , guinea pig.
Journal title :
Pakistan Journal Of Pharmaceutical Sciences
Journal title :
Pakistan Journal Of Pharmaceutical Sciences