Author/Authors :
Nadeem, Qaisar University of the Punjab - Institute of Chemistry, Quaid-e-Azam Campus, Pakistan , Khan, Irfan Ullah Institute of Nuclear Medicine Oncology (INMOL) - Radiopharmacy Division, Pakistan , Javed, Muhammad Gujranwala Institute of Nuclear Medicine (GINUM), Pakistan , Mahmood, Zaid University of the Punjab - Institute of Chemistry, Quaid-e-Azam Campus, Pakistan , Dar, Ume-Kalsoom Institute of Nuclear Medicine Oncology (INMOL) - Radiopharmacy Division, Pakistan , Ali, Muhammad University of the Punjab - School of Biological Sciences (SBS), Quaid-e-Azam Campus, Pakistan , Hyder, Waqar Institute of Nuclear Medicine Oncology (INMOL) - Radiopharmacy Division, Pakistan , Murad, Sohail Gujranwala Institute of Nuclear Medicine (GINUM), Pakistan
Abstract :
N-(2-Hydroxybenzyl)-2-amino-2-deoxy-D-glucose (NHADG) was synthesized by conjugation of salicylaldehyde to glucosamine. The obtained compound was well characterized via different analytical techniques. Labeling of the synthesized compound with technetium-99m (99mTc) in pertechnetate form (99mTcO4 -) was carried out via chelation reaction in the presence of stannous chloride dihydrate. Maximum radiochemical yield of 99mTc-NHADG complex (99%) was obtained by using 1 mg NHADG, 200 μg SnCl2.2H2O, at pH 9.5 and reaction time of 15 min. The radiochemical purity of the 99mTc-NHADG complex was measured by Instant Thin Layer Chromatography (ITLC) and Paper Chromatography (PC), without any notable decomposition at room temperature over a period of 4h. The biological evaluation results show that the 99mTc labeled NHADG conjugate is able to specifically target mammary carcinoma in mice models, thus highlighting its potential as an effective 99mTc labeled glucose-derived agent for tumor imaging.
Keywords :
Synthesis , Technetium , 99m , glucosamine derivative , tumor , imaging
