Author/Authors :
Almeida, Michelle Rodrigues Ayres de Universidade Federal do Rio de Janeiro, Cidade Universitária - Centro de Ciências da Saúde - Núcleo de Pesquisas de Produtos Naturais, Brazil , Leal, Ivana Correa Ramos Pólo Universitário - Faculdade de Farmácia, Brazil , Leal, Ivana Correa Ramos Universidade Federal do Rio de Janeiro - Campus Macaé, Brazil , Ruela, Halliny Siqueira Universidade Federal do Rio de Janeiro, Cidade Universitária - Centro de Ciências da Saúde, Brazil , Araujo, Maria da Graça Justo Universidade do Estado do Rio de Janeiro - Instituto de Biologia Roberto Alcantara Gomes, Brazil , Martins, Thiago Martino Universidade do Estado do Rio de Janeiro - Instituto de Biologia Roberto Alcantara Gomes, Brazil , Coelho, Marsen Garcia Pinto Universidade do Estado do Rio de Janeiro - Instituto de Biologia Roberto Alcantara Gomes, Brazil , Kuster, Ricardo Machado Cidade Universitária, Cidade Universitária - Centro de Ciências da Saúde - Núcleo de Pesquisas de Produtos Naturais, Brazil , Sabino, Kátia Costa Carvalho Universidade do Estado do Rio de Janeiro - Instituto de Biologia Roberto Alcantara Gomes, Brazil
Abstract :
Cancer chemotherapy is an important strategy to treat this leading cause of death worldwide and plants may constitute a source of new antineoplastic agents. This work fractionated the ethanolic extract of Jacaranda puberula leaves and studied the in vitro antitumoral action and some toxicological effects of the most bioactive fraction. Cell lines related to worldwide cancers were used. The Dichloromethane (DCM) and PP fractions were the most bioactive ones. The anti-tumoral action of the DCM fraction was higher than that of the crude EtOH extract while that of PP fraction was higher than the original one (DCM) for both breast (MCF-7), prostate (PC3) and lung (A549) tumor cells, chronic leukemia cells. The K562 cells were the most sensitive cell line. The PP fraction (20 μg/ml) cytotoxicity for these cells was similar to that of the ursolic acid triterpene or the antineoplastic ethoposide. The PP fraction inhibited K562 cell proliferation without cell cycle arrest in a specific phase or apoptosis. PP increased the mitochondrial reduction activity of lymphocytes. After a single dose by oral route, PP fraction did not induce intrinsic acute toxicity or animal death. This work demonstrated that the J. puberula fraction (PP) present high in vitro anti-tumoral effect with no cytotoxicity for immune system cells or oral acute toxicity, improving the Jacaranda puberula ethnopharmacology and reporting new biological effects for the genus Jacaranda.
Keywords :
Jacaranda puberula , Antitumor , Acute toxicity , Pentacyclic triterpenes
