Author/Authors :
Yuan, Huaibo Hefei University of Technology - College of Biotechnology and Food Engineering, China , Yuan, Huaibo Zhejiang University - College of Biosystem Engineering and Food Science, China , Meng, Shaohua Hefei University of Technology - College of Biotechnology and Food Engineering, China , Wang, Guoze Inner Mongolia University of Science and Technology - School of Mathematical Physics and Biology Engineering, China , Wang, Guoze Zhejiang University - College of Biosystem Engineering and Food Science, China , Gong, Zhaobin Hefei University of Technology - College of Biotechnology and Food Engineering, China , Sun, Wenkai Hefei University of Technology - College of Biotechnology and Food Engineering, China , He, Guoqing Zhejiang University - College of Biosystem Engineering and Food Science, China
Abstract :
The antioxidant effects of the triterpenoid-rich extracts from Euryale ferox shell (ES) have been confirmed in vitro. This study examined whether the triterpenoid-rich extract from ES eases human hyperglycemia and diabetes caused by metabolic disorders. Normal and streptozocin (STZ)-induced diabetic mice were used as controls for the four groups that received the triterpenoid-rich extracts of ES suspended in distilled water orally at doses of 200, 300, 400, 500±2 mg/L. Body weight, blood glucose and pancreatic tissue morphology were observed after 4 weeks. The expression of protein tyrosine phosphatase-1B (PTP1B) and insulin receptor substrate (IRS-1) proteins, which are related to the regulation of glucose metabolism in vivo, were also investigated. Compared with the model group (LD50 900±2 mg/L), it was found that the triterpenoid-rich extracts of ES could regulate glucose metabolism (P 0.01) and cause body weight to return to normal levels (P 0.05). Islet morphology recovered well, the expression of the negative regulation protein PTP1B gene was reduced and insulin receptor IRS-1 protein expression was increased. These data prove that the triterpenoid-rich extracts from ES have a therapeutic effect on diabetes by insulin resistance.
