Effect of Auxin derivatives on morphological and isoenzyme pattern of enzymatic Antioxidant peroxidase (POX) of “blinding eye mangrove” Excoecaria agallocha.L stem cuttings.

In our investigation, the effect of auxin on rooting and sprouting behavior of stem cutting of Excoecaria agallocha has been studied. Initially stem cuttings were pretreated to remove the phenol content in the cutting and then they stem were subjected to hormonal treatment with auxins derivatives as IBA 2000 ppm, IPA 2000 ppm, NAA 2000 ppm, and IBA+NAA combination 2000 ppm. The root length and their number, rooting and sprouting percentage, number of leaves per cutting, leaf area, and photosynthetic pigments were analyzed on days 40, 50, and 60 after planting (DAP). Also the isoenzyme pattern of peroxidase of root and leaf were analyzed. Among the auxin treatments, IBA 2000ppm vastly enhanced rooting and sprouting behavior followed by IPA, IBA+NAA, and NAA of Excoecaria agallocha. The isoenzyme analysis for peroxidase clearly showed that peroxidase (POX) highly supported both root initiation and elongation processes in Excoecaria agallocha L.