Passive immunization against Pseudomonas aeruginosa Infected in burn wound in mice

The present study aimed to know the efficacy of a ntisera of Pseudomonas aeruginosa and Salmonella typhimurium in protect burn mice against Pseudomonas aeruginosa infection. Twenty mice have been used (6-8 weeks age) and exposed to burn wound by hot water (1.5x1.5cm2, 900C). After 24hrs, the animals were divided randomly into four groups equally. The 1st, 2nd and 3rd groups were inoculated with 0.3ml of bacterial suspension contain 1x109 cfu/ml, under the burn area. Immediately, the 1st and 2nd groups were inoculated 1/V with 0.1ml of antisera of S.typhimurium and P.aeruginosa respectively. The 4th group was inoculated with 0.3ml of sterile normal saline under the burn area as hegative control group. The results showed that all infected-non-passive immunizaed animals died during24-96hrs post-burn wound infection with heavy bacterial isolation from examined internal organs and sever pathological lesions characterized by neutrophils infiltration in the stroma of the tissues. Congestion of blood vessels with neutrophils in their lumen was seen, in addition to degenerative and necrotic changes as well as thrombus in the blood vessels in some organs while infected-passive immunized animals were survived with good healthy as well as no bacterial isolation and no or mild pathological lesions in their internal organs