Detection of canine parvovirus in Iraq by using rapid antigen test kit and Haemagglutination –inhibition test

This study considered to be the first in Iraq conducted for detection of canine parvovirus from dogs. The virus was detected in pups brought to private Vet. Clinics in Baghdad. Pups suffered from bloody diarrhea, vomiting, dehydration and increased body temperature. Canine parvovirus antigen detected by using rapid antigen test kit and Haemagglutination – inhibition tests ( HI). The result of rapid test showed the ratio of positive samples was 66.6%. The fecal samples were checked for Haemagglutination potential using different types of erythrocyte which include rabbit, chicken, horse and dogs and showed high potential HA activity if this test performed early at the time of sample collection. The values ranging from 16- 512 Haemagglutination units. Serological characterization using of Haemagglutination–Inhibition test by using anticanine parvovirus antibodies which were prepared against standard attenuated vaccine in rabbit which gave titer of 64 Haemagglutination–inhibition . Other fecal samples checked for HI and gave high titer.