In vitro effect of alcoholic extract of Pimpinella anisum against some bacterial growth

Alcoholic extract of Pimpinella anisum fruit have been prepared at different concentration (10, 25, 50, 100, 200) mg/ ml. The extract of plant tested in vitro against (7) bacterial given by disc diffusion method Escherichia coli, Staphylococcus aureus, Proteus vulgaris, Listeria monocytogenes, Salmonella typhimurium, Pseudomonas aeruginosa and Klebsiella pneumonia. The result have been shown that the effect of extract at concentration of 200 mg/ml gave most inhibition zone on Pseudomonas aeruginosa (18) mm, and less than in case of Salmonella typhimurium (17) mm, Staphylococcus aureus (14) mm, Escherichia coli (13) mm, Klebsiella pneumonia (9) mm. At concentration (100) mg/ml, the growth inhibition zone was (12) mm in case of Pseudomonas aeruginosa, Staphylococcus aureus (10) mm, and Escherichia coli (10)mm. At concentration 50 mg/ml inhibition zone was 9 mm for Staphylococcus aureus, Escherichia coli 10 mm, while for concentration of 25 and 10 mg/ml the inhibition zone was (8), (10) mm for Staphylococcus aureus growth respectively.