Two different diagnostic methods for detection of rotavirus in Iraqi young Childreen

ELISA (Premier Rotaclone) was compared with latex agglutination test (LT) (Bio Kit) for detection of Rota virus in fecal samples from clinically suspected cases of viral gastroenteritis in children. Out of 40 samples 12(30%) were positive for Rota virus antigen by ELISA kit. While 30 samples (75%) samples were positive for Rota virus by latex agglutination test (LT). All controls were negative for viral antigen by ELISA and LT test. ELISA and Latex kits were found to be economically sensitive for screening and rapid diagnosis of rota virus diarrhea. In conclusion, our study showed that the latex agglutination is clearly a reliable and rapid method for detection rotavirus but Elisa is more sensitive than the latex agglutination.