Adaptive mutation through exposure to organic and inorganic biocides lead to the antibiotics resistance

In this study we show the effect of some of biocides on Acinetobacter baumannii and reflection of this effect on resistance of bacteria to both biocides and antibiotics at the same time. We have Used inorganic biocide which represent Sodium hypochlorite compound (NaOCl). Exposure of bacteria to this compound in sub-lethal concentrations are 400 μgml (0.04%) and 500 μgml (0.05%). Also bacteria are exposed to organic biocide which represent Povidone-iodine (PVP-I) exposure of bacteria to concentrations 2000 μgml (0.2%) and 3000 μgml (0.3%), after that we observe the appearance of bacterial strains resistant to biocides by observation of increase in Minimal Inhibitory Concentration (MIC) values for biocides to inhibit the bacteria after exposure it to biocides in the concentration which are mentioned above. We observed from this study that clear differences in bacterial susceptibility to antibiotics before and after exposure by observation increase in the level of bacterial resistance to antibiotics after exposure to biocides. We conclude from this study we must know the appropriate concentrations for these biocides (Sodium hypochlorite and Povidone iodine) because if we use these biocides in sub-lethal concentration lead to appearance of bacterial strains resistant to both biocides and antibiotics and that lead to failure in the treatment.