Abstract :
A study were carried out to investigate, the immunomodulatory effect of the Mannoproteins of Candida albicans cell wall on the immune response of mice vaccinated with Brucella RB51 vaccine and considered as biological immunomodulators. The study included two main groups, each group divide into four subgroups, each subgroup involved 20 mice, the first four subgroups were I: treated with distilled water. II: treated with the Brucella RB51 vaccine only III: treated with the mannoproteins only. IV: treated with brucella-RB51 strain vaccine and mannoproteins of Candida albicans. The V, VI, VII and VIII groups were injected with the immunosuppressive drug prednisolone prior to the forthcoming treatment. All these treatments were carried out on day 1, then the mice were sacrificed on day 8 to estimate serum IFN-γ level and on day I4 for delayed-type hypersensitivity reaction. The doses of the Mannoproteins of Candida albicans cell wall represents 10% of the calculated LD50 dose (5714 μg/kg), which were given subcutaneously. The results demonstrated a clear immunomodulatory effects of the mannoproteins of Candida albicans cell wall (improvement of non-specific, and cellular immune response) of the treated mice vaccinated with BrucellaRB51. The interferon-γ (IF- γ) showed a significant increased (P≤0.05) in serum level in Mannoproteins-treated vaccinated mice in comparison with negative and positive subgroups, and group IV showed a highest increase. In delayedtype hypersensitivity reaction, an increased index was significantly increased (P≤0.05) in Mannoproteins-treated vaccinated mice in comparison with negative and positive subgroups, a best results was observed after 24 hours post-brucelline injection, generally the response after 24 hours was better than 48 hours.
