Effect of lipopolysaccharide on the liver function test

Lipopolysaccharide (LPS; endotoxin), a major component of the outer membrane of the Gram-negative bacterial cell wall. Among the changes observed upon exposure to LPS are fever, circulatory shock, disseminated intravascular coagulation, and damage to numerous organs including the liver, acute lung injury and acute renal failure. 40 male Wister albino rats were divided in to 3 groups. Control group was given tap water during the experiment period. At the last day of the experiment, animal were injected intraperitoneally with distilled water. The second group was given ethyl alcohol with drinking water, at the end of the experiment 10 rats were injected intraperitoneally with distilled water and the other 10 rats were injected intraperitoneally with LPS (1μg/gm). Endotoxin group was given tap water during the experiment period, at the last day of the experiment period they were injected intraperitoneally with LPS (1μg/gm). for determining the biochemical tests the blood were collected in test tubes for obtaining the serum samples. Injection of LPS significantly increased the activity of alkaline phosphatase enzyme (SALP) and bilirubin level in serum as well as serum glutamic oxaloacetic transaminase (SAST) and glutamic pyruvic transaminase (SALT) were also increased compared with control rats. LPS significantly increased (SAST), (SALT), the activity of alkaline phosphatase enzyme (SALP) and bilirubin level in serum and it’s regarded as a natural results of organs damage and dysfunction which caused by LPS insult.