Isolation and characterization of phenazine produced from mutant

Hundred and four isolates of Pseudomonas aeruginosa were screening and isolate SP9 identified as belong to Pseudomonas aeruginosa, and was selected according to it s high phenazine production. That isolate was subjected to mutation by different concentrations of N-methyl-N-nitro-N-nitrosoguanidine (NTG). In 250 μg/ml of NTG, the mutant strain produced noticeable amount of phenazine more than isolate of strain SP9 which named SPm9. separation and characterization of phenazine was done by using TLC, HPLC and IR spectrum. Gel filtration was used for purification of phenazine with sephadex G25. Antimicrobial activity of phenazine was done by using disk method for numbers of Gram-positive and Gram-negative isolates. The results indicate that phenazine which produced from mutant strain SPm9 is a broad spectrum antibiotic follows like that produced from isolate strain Sp9. it is effective against Bacillus subtillus, Staphylococcus aureus and Staphylococcus epidermidis as well as Escherichia coli and Salmonella typhi. Phenazine was also found to be effective against Candida albicans.