Author/Authors :
RADJASA, OCKY KARNA Diponegoro University - Center for Tropical Coastal and Marine Studies - Department of Marine Science, Indonesia , MARTENS, TORBEN University of Oldenburg - Insitute for Chemistry and Biology of the Marine Environment, Germany , GROSSART, HANS-PETER University of Oldenburg - Insitute for Chemistry and Biology of the Marine Environment, Germany , SABDONO, AGUS Diponegoro University - Center for Tropical Coastal and Marine Studies - Department of Marine Science, Indonesia , SIMON, MEINHARD University of Oldenburg - Insitute for Chemistry and Biology of the Marine Environment, Germany , BACHTIAR, TONNY Diponegoro University - Center for Tropical Coastal and Marine Studies - Department of Marine Science, Indonesia
Abstract :
A coral-associated bacterium was successfully screened for secondary metabolites production based on PCR amplification of the nonribosomal peptide synthetase gene and was identified as closely related to Pseudoalteromonas luteoviolacea based on its 16S rDNA.The bacterium was found to inhibit the growth of shrimp pathogenic bacterium tested, Vibrio harveyi. To characterize the inhibiting metabolite, a 279 bp long DNA fragment was obtained and the deduced amino acid sequence showed conserved signature regions for peptide synthetases and revealed a high similarity to NosD (40% identity), a multifunctional peptide synthetase from Nostoc sp. GSV224, and NdaB (44% identity), a peptide synthetase module of Nodularia spumigena.
