In Vitro Synergistic enhancement of Newcastle Disease Virus to Methotrexate cytotoxicity against tumor cells

Combination chemotherapy is a common treatment for cancer patient. In the recent years there is a new type of combination had been introduced to the clinical field, that by combine chemotherapy with virotherapy, Using oncolytic viruses. Newcastle disease virus is one of the most important oncolytic viruses and has been introduced to clinical field, and showed no serious side effects. In the present study, Newcastle disease virus combined with one of the most common chemotherapy used extensively in cancer patients, Methotrexate (MTX). Study results showed synergistic effect of NDV with MTX in the four cell line tested, Hep-2, RD, AMN3 and Vero cell lines. Combination therapy was always more effective than chemotherapy alone or virotherapy alone. This study suggests presence of synergistic action between MTX and NDV against tumor cells in vitro, which may give a new hope to stand against cancer.