Therapeutic effect of cold extractethanolplantago Lanceolata leaves ointment in induced infected burn-wound in mice

Medicinal plants have been reported to be very beneficial in wound-burn care, this study to evaluate the effect of topical application of plantago lanceolata ointment comprising from dried cold alcoholic extract of plantago lanceolata leaves 5 gm and95gm of pure Vaseline on the healing of incisional wounds and burn contamination with 105 CFU/ml of staphylococcus aureus and 1.5X108 CFU/ml of Pseudomonus aeruginosa in mice, this treatment divided in two steps, first step invitro experiment of extract effect on bacterial growth and second step invivo on dorsal back of 12 mice in two sites left and right back site which divided in two equal group (group A and group B) which subdivided in two groups (3 mice for burns -3 mice for wound) group A contamination with staph. aureus and group B contamination with Pseudomonus aeruginosa, has been investigated the effect of plantago lanceolata ointment on woundburn healing were critically dependent on the time of application after wounding and burning, topical treatment starting at 2 day after wound- burn contamination, results showed significantly inhibition effect of plantago lanceolata cold extract in growth of bacteria, so optimal effects on improving wound- burn healing were observed plantago lanceolata ointment with concentration 5% significantly increased the rate and extent of wound- burn healing and inhibition growth of bacteria at 7-20 days after treatment compared with control sit of wound- burn which contamination with bacteria without treatment where healing appearance in 26 days of wound- burn infection. This suggest that plantago lanceolata ointment is effective improving wound- burn healing against contamination with bacterial infection as a back ground and it’s important to investigate these herbal ointments activities without side and toxic effects. So short of treatment period treatment compared with classical case of wounds and burns.