Author/Authors :
ashrafi, a. shiraz university of technology - department of chemistry, Shiraz, Iran , alavianmehr, m.m. shiraz university of technology - department of chemistry, Shiraz, Iran , mohammad-aghaie, d. shiraz university of technology - department of chemistry, Shiraz, Iran , yousefi, r. shiraz university - protein chemistry laboratory (pcl), department of biology, Shiraz, Iran , golbon haghighi, m. shahid beheshti university - department of chemistry, Tehran, Iran , moosavi-movahedi, a.a. university of tehran - institute of biochemistry and biophysics (ibb), Tehran, Iran , soltani rad, m.n. shiraz university of technology - department of chemistry, Shiraz, Iran
Abstract :
Mutual interactions of human serum albumin (HSA) with the two binuclear platinum complexes, containing [Pt2Cl2(bhq)2(μ-dppm)] (1) and [(p-MeC6H4)(bhq)Pt(μ-dppm)Pt(bhq)(CF3CO2)] (2), in which bhq = benzo[h]quinoline, and dppm = bis(diphenylphosphino) methane, were thoroughly investigated using spectroscopic and molecular modeling techniques. In this respect, fluorescence, Ultraviolet-visible (UV-Vis) and circular dichroism (CD) spectroscopies, along with the docking and molecular dynamics simulations (MD) were utilized. Analysis of spectroscopic and MD simulation results revealed the structural alterations of HSA upon binding to the binuclear platinum complexes, while the hydrogen bonding and van der Waals forces were found to mainly contribute to the protein-ligand intermolecular interactions. Results of far-UV CD measurements showed the strong ability of platinum complexes in reducing the α-helical content of HSA, while increasing the other secondary structural features. Due to their different chemical natures, these complexes bind to HSA in different manners. Binding constants and thermodynamic binding parameters between these complexes and HSA were calculated using the Stern-Volmer and van’t Hoff equations. Calculated thermodynamic binding parameters indicated that the interaction is spontaneous and enthalpy driven, through the static and dynamic quenching mechanisms, for complexes 1 and 2, respectively.
Keywords :
Protein , ligand interaction , HSA , Platinum complex , Fluorescence spectroscopy , Molecular dynamics simulation
