Histopathological study in liver and spleen of mice infected with Brucella melitensis

Brucellosis is a chronic infectious disease caused by Brucella spp.,a Gram-negative facultative intracellular pathogen that affects humans and animals, leading to significant impact on public health and animal industry. The mouse is the animal model more commonly used to study chronic infection caused by Brucella. This model is most frequently used to investigate specific pathogenic factors of Brucella spp. This work was done to study the histopathological in liver and spleen in mice infected with Brucella melitensis. A total of 20 female mice (8 week of age) a 10 mice control and 10 injected with 105cfu (colony forming unit) of Brucella melitensis by injection intraperitonial per animal. Samples (liver and spleen) were collected at 6 weeks period of infection and kept in 10% formalin study which revealed congestion, granulomatous , fibrosis in liver and increased number of lymphohistiocytic cells and increased amount of red pulp in spleen. This study concluded that the histopathological in liver and spleen caused by Brucella melitensis in mice are similar to those observed in humans with brucellosis, which indicated that the mice are suitable model for histopathological studies of human being diseases especially brucellosis