Genome pattern of Salmonella Enteritidis from reproductive organs and eggs from Egyptian laying hens

The present study sought to determine whether Salmonella enteritidis isolated from freshly laid eggs and Salmonella colonized-reproductive tissues of naturally infected hens had same genome pattern. Ninety layer hens from three different farms (coded farm-1, -2 and -3) were used as following: 30 from farm-1, 25 from farm-2 and 35 from farm-3). In addition to 145 laid eggs randomly collected from the same farms. Both tissue samples and Eggs were examined for presence of Salmonella by bacteriological, serological and molecular methods. The results revealed 8 out of 90 hens were positive for genus Salmonella. Salmonella enteritidis was identified based on OMPC and ENT genes-based Multiplex PCR. Determination of strain specific pattern of isolated Salmonella was carried out by ERIC test. Out of 90 layer hens tissue organs, Salmonella Enteritidis detected in ovaries, oviduct, forming eggs and intestine of layer hens were 5, 2, 1 and 7 cases respectively. Out of 145 laid eggs, Salmonella Enteritidis was detected in 2 and 1 of outer egg shell and internal eggs contents respectivlly. That ″hot strain″ infected the ovary, intestine, forming egg of naturally infected laying hens proved to have unique DNA alles by ERIC- PCR.