Expression Pattern of Some Immune Response Genes in Bovine Mastitis Milk

Mastitis is the most costly disease for dairy farmers and industry, which are mainly ensued by the entry of bacteria of the teat canal. Shortly after the entry of the invading bacteria, the innate immunity recognizes the invading pathogen and initiates the inflammatory response. This initial inflammatory response release cytokines and chemo attractants for the rapid and massive influx of neutrophils from the blood to the site of infection which form the first line of cellular defense against bacteria. In order to characterize the expression of gene associated with immune response mechanism to mastitis we quantified the relative expression of the IL-4, IL-6, IL-8 and IL-10 genes in milk somatic cells of cows with clinical mastitis. The mRNA abundance of a target gene was normalized with housekeeping gene (B-Actin) and expressed as fold of induction. Result revealed that up regulation of all target cytokine genes in response to infection with S.aureus. These finding suggest that animals with mastitis develop a preferentially cell which mediated immune response, further study including number of samples are necessary to better characterize the gene expression profile in caws with S. aureus mastitis .