Title of article :
Molecular Characterization of Some Egyptian cotton Varieties
Author/Authors :
saif, issraa alexandria university - faculty of agriculture - department of genetics, Alexandria, Egypt , seehy, m.a alexandria university - faculty of agriculture - department of genetics, Alexandria, Egypt , riad, sanaa cotton arbitration and testing general organization (catgo), Egypt , elbagoury, mahmoud cotton arbitration and testing general organization (catgo), Egypt
Abstract :
Cotton plant belongs to the genus Gossypium which is considered as the first important economic crop in Egypt. The present study aims to characterize five Egyptian cotton varieties (Giza(G) 92;Giza(G) 93; Giza(G)86; Giza(G)87and Giza(G)88) at biochemical and molecular level. Using ISSR; SSR and RAPD-PCR analyses, a total of 159 polymorphic fragments were recorded with similarity of 40; 23.5 and 38%, respectively, for the five varieties. The Phylogenetic relationships of the five Egyptian cotton varieties based on DNA molecular data (RAPD + ISSR + SSR) indicated two groups with 56% similarity; the first group had (G88); However, G93 and G86 with homology percentage of 81%, and both of them in relation to G87 variety with approximately 65.5% similarity.
Keywords :
Cotton , Molecular Markers , ISSR , SSR , RAPD , PCR
Journal title :
alexandria science exchange journal
Journal title :
alexandria science exchange journal
