Author/Authors :
Kokina, Inese Daugavpils University - Institute of Systematic Biology, LATVIA , Sledevskis, Eriks Daugavpils University - G Liberts Innovative Microscopy Centre, LATVIA , Gerbreders, Vjaceslavs Daugavpils University - G Liberts Innovative Microscopy Centre, LATVIA , Grauda, Dace University of Latvia - Institute of Biology, LATVIA , Jermalonoka, Marija Daugavpils University - Institute of Systematic Biology, LATVIA , Valaine, Kristina Daugavpils University - Institute of Systematic Biology, LATVIA , Gavarane, Inese Daugavpils University - Institute of Systematic Biology, LATVIA , Piginka, Inga Institute of Food Safety - Animal Health and Environment “BIOR”, LATVIA , Filipovics, Maksims Daugavpils University - Institute of Systematic Biology, LATVIA , Rashal, Isaak University of Latvia - Institute of Biology, LATVIA
Abstract :
Frequency of callusogenesis, type and capacity of regeneration, cell ploidy, DNA methylation level, histological features, autofluorescence and photobleaching effects were studied in flax calli grown on medium supplemented with different concentrations of carbon (C) nanoparticles. The presence of carbon nanoparticles on medium decreased the percentage calli formation. Detailed histological study led to better understanding of changes in calli caused by nanoparticles. Ploidy variation in calli cells significantly depended on carbon nanoparticle concentration in the medium. Different types of calli regeneration associated with different C nanoparticles concentration in medium were observed: somatic embryogenesis (direct or indirect) or organogenesis. C nanoparticle dose-dependent DNA hypermethylation in flax calli cells were found. Increased repair ability during laser irradiation was found in calli grown on medium with carbon nanoparticles.
Keywords :
flax , calli , cell ploidy , DNA methylation , somatic embryo , histology , autofluorescence , photobleaching
