Title of article
EXPRESSION OF GA COAT PROTEIN-DERIVED MOSAIC VIRUS-LIKE PARTICLES IN Saccharomyces cerevisiae AND PACKAGING in vivo OF mRNAs INTO PARTICLES
Author/Authors
Strods, Arnis Biomedical Research and Study Centre, LATVIA , Argule, Dagnija Biomedical Research and Study Centre, LATVIA , Cielens, Indulis Biomedical Research and Study Centre, LATVIA , Jackevica, Ludmila Biomedical Research and Study Centre, LATVIA , Renhofa, Regina Biomedical Research and Study Centre, LATVIA
From page
234
To page
241
Abstract
Our previous research showed that the best yield of virus-like particles (VLPs) formed by RNA bacteriophage GA coat protein was obtained by expression in yeast Pichia pastoris, while other used expression systems in Saccharomyces cerevisiae gave much lower amounts of capsids. The main reasons to attempt further studies in Saccharomyces cerevisiae were to improve the yield of GA-based VLPs using constructs with optimised nucleotide triplets in coding sequences, and to exploit the possibilities of the two-promoter Gal1/Gal10 system of expression vector pESC-URA for production of the desired mosaic VLPs and for packaging of mRNAs into VLPs in vivo.
Journal title
Proceedings Of The Latvian Academy Of Sciences Section B Natural Exact and Applied Sciences
Journal title
Proceedings Of The Latvian Academy Of Sciences Section B Natural Exact and Applied Sciences
Record number
2657092
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