مرکز منطقه ای اطلاع رساني علوم و فناوري - The effect of irrigation with EDTA on calcium-based root canal sealers: a SEM-EDS and XRD study

Author/Authors :

harik, randa lebanese university - school of dentistry - department of endodontics, Lebanon , salameh, ziad lebanese university - school of dentistry - department of research, Lebanon , salameh, ziad university of pittsburgh - center for craniofacial regeneration, USA , habchi, roland lebanese university, pierre gemayel campus - faculty of science ii, Lebanon , camilleri, josette university of malta - faculty of dental surgery, medical school - department of restorative dentistry, Malta

Abstract :

Introduction: Calcium silicate-based root canal sealers interact with dentine through reaction of calcium hydroxide with dentinal fluids. Ethylenediaminetetraacetic acid (EDTA), a calcium chelator, is used as the final irrigant for smear layer removal. EDTA presence in root canal may potentially affect the interaction of calcium silicate–based sealers with dentine. The aim of this study was to investigate the effect of EDTA final rinse during root canal therapy on the chemical composition and interaction of tricalcium silicate-based sealers with dentine. Methodology: Tricalcium silicate-based root canal sealers MTA Fillapex, EndoSequence BC sealer, BiorootTM RCS, and a calcium hydroxide-based sealer, Apexit Plus, were investigated. AH Plus was used as control. Roots standardized to 14 mm were debrided and shaped with ProTaper instruments. Final rinse with EDTA, then water, was followed by filling the root canals with the different sealers. After immersion in physiological solution for 28 days, the roots were split and the sealers were characterized by scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), and X-ray diffraction analysis (XRD). Results: A final rinse with water resulted in the uptake of phosphate from the storage solution through dentinal tubules leading to calcium phosphate phase formation in Bioroot RCS and Apexit Plus. The calcium phosphate phase formation could not be verified for EndoSequence BC sealer as this phase was already present in the material. Rinsing with EDTA led to depletion of calcium ions and elimination of the calcium phosphate phase in EndoSequence BC and Apexit Plus sealer. Conversely, calcium phosphate phase formation was demonstrated with MTA Fillapex after rinsing with EDTA. Conclusions: The use of EDTA final irrigation during root canal therapy affects the interaction of tricalcium silicate-based sealers used to obturate the root canal with dentine. The changes observed were dependent on the chemical composition of the sealer.