STIMULATORY EFFECT OF NAA (NAPHTHALENE ACETIC ACID) AND BAP (BENZYL AMINOPURINE)ON FLOWERS, SEEDS, CHLOROPHYLL AND PROTEIN-CONTENT IN SPINACH (SPINACIA OLERACEA L.)

The current research work was conducted at the Faculty of Biological sciences, Quaid-i-Azam University, Islamabad during the year 2006. The study was based on to apply the growth regulators (NAA and BAP) in preflowering and flowering stages to estimate their effects on seed formation, seed setting, chlorophyll and protein contents in spinach (Spinacia oleracea L.). Highly significant value was concluded for number of bolts per plant through NAA at 10^-3M concentration. Non-significant result was obtained in case of treatment for seed yield although maximum seed yield was recorded with concentraion of BAP (10^-3M) and NAA (10^-4M). At the same time, seed weight was noted as significantly increased; whereas maximum seed weight was achieved for NAA 10^-5M. The present research work indicated that chlorophyll content was not affected by the application of BAP and NAA at different combinations and concentrations. For protein analysis the treatments in which NAA and BAP were applied in combination showed maximum variability in seed protein electrophoratic banding profile.