Author/Authors :
GUO, Yue School of Medicine - Huzhou University, Huzhou, China , ZHOU, Hong Chang School of Medicine - Huzhou University, Huzhou, China , DONG, Ying School of Medicine - Huzhou University, Huzhou, China , DONG, Hai Yan School of Medicine - Huzhou University, Huzhou, China , YAO, Yun Liang School of Medicine - Huzhou University, Huzhou, China , QIAN, Jing School of Medicine - Huzhou University, Huzhou, China , ZHANG, Hui School of Medicine - Huzhou University, Huzhou, China , LI, Xiao Yu School of Medicine - Huzhou University, Huzhou, China , ZHANG, Zhong Shan Huzhou University, Huzhou, China , LIN, Han Bing School of Medicine - Huzhou University, Huzhou, China , ZHOU, Tian School of Medicine - Huzhou University, Huzhou, China , ZHAO, Meng Jia School of Medicine - Huzhou University, Huzhou, China , JI, Tang Qin School of Medicine - Huzhou University, Huzhou, China , WANG, Run Ze School of Medicine - Huzhou University, Huzhou, China , ZHANG, Feng Ping School of Medicine - Huzhou University, Huzhou, China
Abstract :
Background: When Angiostrongylus cantonensis develops from the third and fourth stage, it needs to change its host from the middle host, snail to the final host, rat. However, the mechanism involved in this change remains unclear.
Methods: The transcriptome differences of the third and fourth stages of A. cantonensis were explored by next-generation Illumina Hiseq/Miseq sequencing in China, in 2018.
Results: Overall, 137 956 488 clean reads and 20 406 213 373 clean bases of the two stages larvae were produced. Based on the queries against the Gene Ontology (GO), NCBI non-redundant protein sequences (Nr), Swissprot, and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases, 14 204 differentially expressed genes (DEGs) were predicted. GO enrichment analysis revealed 5660 DEGs with the top s categories as followings: biological process (GO:0008150, related to 5345 DEGs), cellular component (GO:0005575, related to 5297 DEGs), molecular function (GO:0003674, related to 5290 DEGs). In KEGG enrichment analysis, 116 genes were related to oxidative phosphorylation and 49 genes involved in the glycolytic process.
Conclusion: Metabolism changes, especially oxidative phosphorylation and glycolysis, might play a key role in A. cantonensis infection of its final rat host. Many other pathways might also contribute to the transcriptome changes between these two life stages. Overall, additional studies are needed for further details.
