Author/Authors :
Javadzahed, Taban Department of Microbiology and Virology - Faculty of Medicine - Mashhad University of Medical Sciences, Mashhad, Iran , Gharib, Masoumeh Department of Pathology - Faculty of Medicine - Mashhad University of Medical Sciences, Mashhad, Iran , Khadem-Rezaiyan, Majid Mashhad University of Medical Sciences, Mashhad, Iran , Meshkat, Zahra Department of Microbiology and Virology - Faculty of Medicine - Mashhad University of Medical Sciences, Mashhad, Iran , Amel-Jamehdar, Saeed Department of Microbiology and Virology - Faculty of Medicine - Mashhad University of Medical Sciences, Mashhad, Iran , Kalantari, Mahmoud-Reza Department of Pathology - Faculty of Medicine - Mashhad University of Medical Sciences, Mashhad, Iran , Jahanbakhsh-kotlar, Hasan Faculty of Medicine - Mashhad University of Medical Sciences, Mashhad, Iran , Bakhshi, Tahmine Antimicrobial Resistance Research Center - Mashhad University of Medical Sciences, Mashhad, Iran , Youssefi, Masoud Department of Microbiology and Virology - Faculty of Medicine - Mashhad University of Medical Sciences, Mashhad, Iran
Abstract :
Background and Aims: The BK virus a member of the Polyomaviruses family was first isolated from the urine of the kidney recipient. Infection with this virus and infection usually occurs in childhood [5-9 years] but most of the time [90%] of sera are positive and without symptoms. Polyomaviruses including the BK virus have also been suggested to be a contributing factor to some cancers in humans such as brain tumors, bone tumors, Kaposi’s sarcoma, adrenal tumors, renal carcinoma, prostate cancer, urinary tumors, genital tumors etc. However, the topic still remains controversial.
Materials and Methods: In this study, we report the presence of BK specific DNA sequences in bladder cancer by PCR and Nested PCR methods.
Results: Our study results confirmed the presence of BK in 13.7% of the samples by Nested PCR method.
Conclusion: In conclusion, 51 samples 13.7% of paraffin-embedded bladder cancer sample were confirmed by Nested PCR method.
Keywords :
Seroprevalence , Infectious Laryngotracheitis , Broiler , Iran , ELISA
