Author/Authors :
Hejazian, Leila Beigom tehran university of medical sciences tums - school of medicine - department of anatomy, تهران, ايران , Hejazian, Leila Beigom babol university of medical sciences - School of Medicine - department of anatomy, ايران , Esmaeilzade, Banafshe bushehr university of medical sciences - school of medicine - department of anatomy, ايران , Moghanni Ghoroghi, Fatima tehran university of medical sciences tums - school of medicine - department of anatomy, تهران, ايران , Moradi, Fatemeh tehran university of medical sciences tums - school of medicine - Department of Anatomy, تهران, ايران , Hejazian, Marzieh Beigom payame noor university - department of physical education, تهران, ايران , Aslani, Anahita tehran university of medical sciences tums - rassoul akram hospital, minimally invasive surgery research center, تهران, ايران , Bakhtiari, Mehrdad tehran university of medical sciences tums - school of medicine - Department of Anatomy, تهران, ايران , Soleimani, Masoud tarbiat modares university - school of medicine - Department of Hematology, تهران, ايران , Nobakht, Maliheh tehran university of medical sciences tums - school of medicine, anti-microbial resistance research center - department of histology and neuroscience, تهران, ايران , Nobakht, Maliheh tehran university of medical sciences tums, تهران, ايران
Abstract :
Background: The aim of this study was to fabricate the poly caprolactone (PCL) aligned nanofiber scaffold and to evaluate the survival, adhesion, proliferation, and differentiation of rat hair follicle stem cells (HFSC) in the graft material using electrospun PCL nanofiber scaffold for tissue engineering applications. Methods: The bulge region of rat whisker was isolated and cultured in DMEM: nutrient mixture F-12 supplemented with epidermal growth factor. The morphological and biological features of cultured bulge cells were observed by light microscopy using immunocytochemistry methods. Electrospinning was used for production of PCL nanofiber scaffolds. Scanning electron microscopy (SEM), 3-(4, 5-di-methylthiazol- 2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay, and histology analysis were used to investigate the cell morphology, viability, attachment and infiltration of the HFSC on the PCL nanofiber scaffolds. Results: The results of the MTT assay showed cell viability and cell proliferation of the HFSC on PCL nanofiber scaffolds. SEM microscopy images indicated that HFSC are attached, proliferated and spread on PCL nanofiber scaffolds. Also, immunocytochemical analysis showed cell infiltration and cell differentiation on the scaffolds. Conclusion: The results of this study reveal that PCL nanofiber scaffolds are suitable for cell culture, proliferation, differentiation and attachment. Furthermore, HFSC are attached and proliferated on PCL nanofiber scaffolds.
Keywords :
Electrospinning , Nanofiber , Stem cells , Tissue engineering
