Author/Authors :
REZAIEYAZDI, ZAHRA mashhad university of medical sciences - Department ofRheumatology, مشهد, ايران , TAVAKKOL AFSHARI, JALIL mashhad university of medical sciences - Department of Immunogenetic and Tissue Culture, مشهد, ايران , ESMAILI, EHSAN mashhad university of medical sciences - Department of Immunogenetic and Tissue Culture, مشهد, ايران , OROUJI, ELYAS mashhad university of medical sciences - Department of Immunogenetic and Tissue Culture, مشهد, ايران , PEZESHKPOUR, FAKHROZAMAN mashhad university of medical sciences - Department ofDermatology, مشهد, ايران , KHODADOOST, MOHAMMAD mashhad university of medical sciences - Department of Immunogenetic and Tissue Culture, مشهد, ايران , MAZHANI, MARYAM mashhad university of medical sciences - Department ofRheumatology, مشهد, ايران , SANDOOGHI, MAHNAZ mashhad university of medical sciences - Department ofRheumatology, مشهد, ايران
Abstract :
Systemic lupus erythematosus (SLE) is an autoimmune disease in which polymorphisms within the human leukocyte antigen (HLA) region have been associated to its etiology. We conducted this study to compare the HLA-DQB1 allelic sequence variation among SLE patients and controls in the northeast of Iran. Genomic DNA of 40 SLE patients and 83 healthy controls were amplified by Polymerase Chain Reaction with Sequence-Specific Primers technique (peR-SSP). Seven serological subclasses of the HLA DQB1 were detected. Allele distribution comparison showed in the SLE group a significant increase of HLA DQ6 (*0601-*0609) (p=0 ()06); whereas alleles HLA DQ7 (*0301-*0304) were significantly decreased (p=0.005). Combination of DQ5 (*0501-*0504)-DQ6 (*0601-*0609) was increased in patients. These results suggest th at DQ6 is the dominant HLA DQB1 allele probably associated with genetic susceptibility to SLE in the northeast of Iran. The association supports the importance of ethnic background and indicates the importance of various genes that has been observed in different SLE populations.
Keywords :
Allelic sequence variation , HLA , DQB1 , Susceptibility , Systemic lupus erythematosus
