Author/Authors :
GAYATHRI, D. Kuvempu University - Department of Microbiology, India , SHANKAR, K.M. Karnataka Veterinary Animal and Fishery Sciences University - College of Fisheries - Department of Aquaculture, Pathology and Biotechnology Laboratory, India , MOHAN, C.V. Network of Aquaculture Centers in Asia-Pacific, Thailand , DEVARAJA, T. N. Taralabalu Krishi Vigyan Kendra (TKVK), India , ANIL, T.M. Karnataka Veterinary Animal and Fishery Sciences University - College of Fisheries - Department of Aquaculture, Pathology and Biotechnology Laboratory, India , KESHAVANATH, P. Karnataka Veterinary Animal and Fishery Sciences University - College of Fisheries - Department of Aquaculture, Pathology and Biotechnology Laboratory, India
Abstract :
A panel of three monoclonal antibodies (MAbs) C1 (IgG1), C13 (IgM) and C14 (IgM) was produced against Aphanomyces invadans for the first time in India. The MAbs C1 and C13 recognized four proteins, while MAb C14 recognized five proteins of the fungus in the Western blot. Specific reaction of the MAbs with the fungus was further confirmed by immunoperoxidase and immunofluorescence where reaction was clearly demonstrated along the hyphae and within granulomas. An epitope analysis of A. invadans in fish from different regions of India, employing present three MAbs by immunoper-oxidase indicated antigen similarity of the said pathogen spread across and in India.
