Title of article :
Inflammatory Response in Indian Major Carp, Catla catla and Barb, Puntius cauveriensis, to Aphanomyces invadans and Freund’s Complete Adjuvant
Author/Authors :
PRADHAN, P.K. Central Agricultural University - College of Fisheries, India , PRADHAN, P.K. Animal and Fisheries Science University - College of Fisheries, Karnataka Veterinary - Department of Aquaculture, Fish Pathology and Biotechnology Laboratory, India , MOHAN, C.V. Network of Aquaculture Centers in Asia-Pacific (NACA), Thailand , MOHAN, C.V. Animal and Fisheries Science University - College of Fisheries, Karnataka Veterinary - Department of Aquaculture, Fish Pathology and Biotechnology Laboratory, India , SHANKAR, K.M. Animal and Fisheries Science University - College of Fisheries, Karnataka Veterinary - Department of Aquaculture, Fish Pathology and Biotechnology Laboratory, India , KUMAR, B.M. University of Guelph - Ontario Veterinary College - Department of Biomedical Sciences, Canada , KUMAR, B.M. Animal and Fisheries Science University - College of Fisheries, Karnataka Veterinary - Department of Aquaculture, Fish Pathology and Biotechnology Laboratory, India
Abstract :
The objective of the present study is to determine the pattern of inflammatory response in two different susceptible fish species Indian major carp, Catla catla and barb, Puntius cauveriensis of Epizootic Ulcerative Syndrome (EUS) to two different antigen models (Aphanomyces invadans and Freund’s complete adjuvant). Inflammatory response studies with A. invadans indicated that in the case of barb there was very weak or no inflammatory response. The severity of myonecrosis was so high that in the moribund fish, virtually no normal muscle fibres were observed in the lesion area. On the other hand, in the case of Indian major carp (catla), there was an active host inflammatory response (particularly at the central part of the lesion) and the severity of myonecrosis was less than that of the barb. Studies on inflammatory response to Freund’s complete adjuvant indicated that similar to A. invadans experiment, the degree of inflammatory cellular infiltration was very low in the case of barb and no inflammatory cells were observed around most of the adjuvant droplets. On the other hand, in the case of Indian major carp, there was a very active inflammatory response and most of the adjuvant droplets were encapsulated by a thick layer of epithelioid cells. Hence, it was considered that due to very poor inflammatory response, the barb might be offering a very insignificant or no resistance to A. invadans infection and that might be one of the major reasons for its high susceptibility to Epizootic Ulcerative Syndrome.
