Title of article :
Study on rapid detection of Vibrio parahaemolyticus with sea food
Author/Authors :
XI-BIN, NING Shanghai Ocean University - College of Food Science, China , YONG, DOU Jiangsu Vocational College of Finance and Economics, China , ER-YI, JU Shanghai Ocean University - College of Food Science, China , DAI-XIN, LIU Shanghai Ocean University - College of Food Science, China
Abstract :
Indirect enzyme-linked immunosorbent assay (ELISA) was developed for the rapid detection of V. parahaemolyticus . Specific polyclonal antibody that produced in New Zealand white rabbits were used in the indirect competitive ELISA.The best reaction concentration of antigen and antibody is 10^7 CFU/ml and 1: 4,000, respectively, and ethe most effective concentration of the secondary HRP-labeled antibody is 1: 1,000. This standardized system was established for detecting V. parahaemolyticus in artificially infected seafood and actual seafood respectively. The detection limit is 10^4 CFU/ml, detection time is 8hours; however, the accuracy could be improved to 10³ CFU/ml after pre-enrichment for 8hours. This system could be of practical application since it was close to the detection result by traditional detection methods.
Journal title :
Asian Fisheries Science Journal (ASJ)
Journal title :
Asian Fisheries Science Journal (ASJ)
