Author/Authors :
CHRISTOPHER, J.G. VIT University - School of Biosciences and Technology, India , MURUGESAN, A.G. Manonmaniam Sundaranar University - Sri Paramakalyani Centre for Environmental Sciences, India , SUKUMARAN, N. Vels University - School of Life Sciences, India
Abstract :
Determination of optimum sperm requirements in artificial breeding helps to im-prove the fertilization efficiency, avoids wastage of sperm and thus minimizes the sacrificing of males for milt. Quantification of sperm cells was done using a spectrophotometer at 420 nm followed by haemocytometer counting. Best correlation (r² = 0.97) was re-corded. The minimum number of sperm cells required for optimal fertilization success in Heteropneustes fossilis was determined. Fertilization success of 78 to 93% was recorded at 8x10³ to 8x10^7 sperm per egg. The highest fertilization success of 98.18% was recorded at 8x10^7 spermatozoa per egg.
