Author/Authors :
ALISHAHI, M. shahid chamran university of ahvaz - Faculty of Veterinary Medicine - Department of Clinical Sciences, Section of Fish Disease, اهواز, ايران , GHORBANPOUR, M. shahid chamran university of ahvaz - Faculty of Veterinary Medicine - Department of Pathobiology, اهواز, ايران , PEYGHAN, R. shahid chamran university of ahvaz - Faculty of Veterinary Medicine - Department of Clinical Sciences, Section of Fish Disease, اهواز, ايران
Abstract :
The effects of oral administration of Viscum album (Family Loranthaceae) and Nigella sativa (FamilyRanunculaceae) extracts on some immune responses of Cyprinus carpio were studied. Four hundred and fifty fish weighing 108±11.4 g were divided into two groups for immune and non-immune treatments. For immune treatment the fish were injected with Aeromonas hydrophila bacterin and the non-immune treatment received sterile phosphate buffer saline. Each treated group was divided into three treatments: Fish in treatments 1 and 2 were fed a diet supplemented with 0.5% (w/w) V. album or N.sativa extracts respectively while in treatment 3 fish were fed a free extract diet. Fish in all treatments were fed for 30 days. Blood samples were taken every 10 days and hematoimmunological indices were compared among treatments. Results showed that oral administration of V. album and N. sativa extracts were able to enhance lysozyme activity, serum bactericidal activity, serum protein and serum globulin in immunised and nonimmunised groups (P 0.05) but complement activity showed no significant change among groups (P 0.05). Resistance to A. hydrophila infection was significantly higher in immunised fish treated with V. album. However, N. sativa did not reduce mortality following the challenge with bacteria. This study indicates that oral administration of V. album extract is a potential candidate for herbal based immunostimulants in common carp.
