Author/Authors :
DESRINA Wageningen University - Aquaculture and Fisheries Group, Netherlands , DESRINA Diponegoro University - Faculty of Fisheries and Marine Science - Study Program Aquaculture, Fisheries Departement, Indonesia , VERRETH, JOHAN A.J. Wageningen University - Aquaculture and Fisheries Group, Netherlands , VLAK, JUST M. Wageningen University - Laboratory of Virology, Netherlands , VERDEGEM, MARC C.J. Wageningen University - Aquaculture and Fisheries Group, Netherlands
Abstract :
The white spot syndrome virus (WSSV) is a highly contagious shrimp pathogen world-wide for which polychaetes are among the many biological vectors. In a previous study WSSV infection was detected in the naturally infected Dendronereis spp. (Nereidae). To further study WSSV infection in polychaetes, a model polychaete that is easy to handle and propagate, and is free of and susceptible to WSSV infection is needed. In the present study the suitability of Hediste diversicolor (Nereidae) was tested as a model animal. WSSV-free H. diversicolor was infected by injection, feeding and immersion, and the infection was followed for 12 days post infection (dpi). In addition, polychaete survival was determined 40 dpi. Hediste diversicolor was able to clear the virus within 4 dpi without showing clinical signs and WSSV-associated mortality. Although a first attempt, it was concluded that H. diversicolor may not be an immediately suitable model animal for WSSV studies in polychaetes.
