Author/Authors :
Servienė, Elena Nature Research Centre, Institute of Botany, Lithuania , Kemežienė, Irena Nature Research Centre, Institute of Botany, Lithuania , Kasperovičienė, Jūratė Nature Research Centre, Institute of Botany, Lithuania , čapukoitienė, Brigita Nature Research Centre, Institute of Botany, Lithuania , Rančelienė, Vida Institute of Botany, Nature Research Centre, Lithuania , Koreivienė, Judita Nature Research Centre, Institute of Botany, Lithuania
Abstract :
The genomic DNA purification method for Gonyostomum semen algae was optimized by applying different DNA purification techniques and rational modifications. This method allowed to obtain high quality DNA preparations suitable for the phylogenetic analysis and genetic variability investigation of algae. DNA isolated by this method yielded strong and reliable amplification products showing their applicability for RAPD-PCR using random decamer primers. In the present study, the RAPD protocol was optimized for the evaluation of Gonyostomum biodiversity.
Keywords :
DNA extraction , algae , invasive raphidophytes , RAPD , PCR analysis.
